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BC-2600 Service Training

Hematology Global Technical Support Department


Service@mindray.com
Version : V2.0
© 2011 Mindray Confidential
NO.:MXQ-12027-BC-2600
OTY-12002 ( 3.0 )
Contents
Principle
1.1 Basic Knowledge
1.2 Principle

BC-2600
2.1 Installation
2.2 Operation
2.3 Liquid System
2.4 Hardware
2.5 System Structure
2.6Trouble Shooting
2.7 Maintenance

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1. Principle

1.1 Basic Knowledge

1.2 Principle

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1.1 Basic Knowledge

Blood Cell Leukocyte Erythrocyte Platelet


Blood

 Blood sample consists of two parts.

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1.1 Basic Knowledge
Blood Leukocyte Erythrocyte Platelet
Blood Cell

 There are three kinds of blood cells.

Leukocyte Erythrocyte Platelet


Size: 30-400fl 30-150fl 2-25fl
9 12 9
Amount: 4-10X10 /L 3.5-5.5X10 /L 100-300X10 /L
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1.1 Basic Knowledge
Blood Blood Cell Erythrocyte Platelet
Leukocyte

Granulocyte
WB
C
Defend the body
against infectious
disease and foreign
materials

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1.1 Basic Knowledge

Blood Blood Cell Erythrocyte Platelet


Leukocyte

Lymph Baso Mono Eosino


Neutr
o

Lymphocyte Mid-sized Cell Neutrophilic Granulocyte


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1.1 Basic Knowledge

Blood Blood Cell Leukocyte Platelet


Erythrocyte

 The function of RBC is almost completed by hemoglobin (HGB).

Transport oxygen and carbon dioxide

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1.1 Basic Knowledge

Blood Blood Cell Leukocyte Erythrocyte


Platelet

Coagulation or clotting

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1. Principle
1.1 Basic Knowledge

1.2 Principle

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1.2 Principle
Electrical Impedance
Method Volumetric Metering Colorimetric Method Reagents

 WBC, RBC and PLT are counted and sorted by the Electrical
Impedance Method, just Coulter Principle. This method is based on the
measurement of changes in electrical impedance produced by a particle
passing through an aperture.

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1.2 Principle
Electrical Impedance
Method Volumetric Metering Colorimetric Method Reagents

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1.2 Principle
Electrical Impedance
Method Volumetric Metering Colorimetric Method Reagents

The amplitude of each pulse is proportional to the size of each blood cell.
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1.2 Principle
Electrical Impedance
Method Volumetric Metering Colorimetric Method Reagents

Pulse Graph 1 、 blood cell of Same size, go through the


aperture by constant speed.

2 、 blood cell of Same size, go through the


aperture by variable speed.

3 、 blood cell of different size, go through


the aperture by variable speed.

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1.2 Principle
Electrical Impedance
Method Volumetric Metering Colorimetric Method Reagents

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1.2 Principle
Electrical Impedance
Method Volumetric Metering Colorimetric Method Reagents

 From Pulse Graph to Histogram

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1.2 Principle
Electrical Impedance Volumetric Metering Colorimetric Method Reagents
Method

From Pulse Graph to Histogram

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1.2 Principle
Electrical Impedance Volumetric Metering Colorimetric Method Reagents
Method

 This analyzer uses a volumetric metering unit to control the count


cycle and to ensure that a precise volume of sample is analyzed.

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1.2 Principle
Electrical Impedance
Volumetric Metering Colorimetric Method Reagents
Method

 HGB is determined by the colorimetric method.


 Lyse will release HGB when RBC was broken down and react with
HGB to generate a mixture for HGB measurement.
 The mixture has the biggest absorbance at 540nm.

I0 It

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1.2 Principle
Electrical Impedance
Volumetric Metering Colorimetric Method Reagents
Method

Absorbing Detector Signal


Light
container processing
source
Display

Photo
Bath sensor
LED light

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1.2 Principle
Electrical Impedance
Volumetric Metering Colorimetric Method Reagents
Method

 Reagents are one part of the system.

 Lyse
Break down RBC;
Release HGB and react with HGB to generate a mixture for HGB
measurement;
Help the differential of WBC.

 Diluent
Dilute the blood sample;
Provide a conductive environment for counting and an environment
which is similar to the plasma to maintain the size of blood cell;
Clean the tube and bath.

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1.2 Principle
Electrical Impedance
Volumetric Metering Colorimetric Method Reagents
Method

 Rinse
Clean the bath and metering tube.

 E-Z cleanser
An enzyme-based cleaning solution used to clean the tube and
bath.

 Probe cleanser
An alkaline cleaning solution used to clean the tube and bath.

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2.BC-2600 Hematology
Analyzer

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2.1 BC-2600 Installation

Installation Requirements
2.1.1 Interface
2.1.2 Installation Requirements
Open box, check 2.1.3 Installation Procedures

Install

Performance validation

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2.1.1 Interface- Front Panel

8.4” color LCD

Keypad

Record

Power indicator
Sample
probe
Start key

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2.1.1 Interface- Left Side

Scanner interface

Printer or upgrade interface

Transmission to computer

Power of floppy disk driver

Keyboard
Printer:
1.EPSON LX-300/300+ ,
2. EPSON LQ-300K+II, LQ630K
3. EPSON Stylus C-87 ,
Panasonic KX-P1150
Scanner: TYSSO CCD-82, AIDA8400
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2.1.1 Interface- Rear Panel

Diluent connector
Rinse connector
Power Switch
Lyse connector
Equipotent Pole Waste connector

Fuse
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2.1.2 Installation Requirements

Environment Space Power

 Operating temperature: 15 ℃ to 30 ℃.
 Relative humidity: 30% to 85%.
 Atmospheric pressure: 70 kPa to 106 kPa.
 free as possible from dust, mechanical vibrations, loud noises, a
nd electrical interference.
 Do not place near brush-type motors, flickering fluorescent lights
, and electrical contacts that regularly open and close.
 Do not place in direct sunlight or in front of a source of heat or dr
afts.

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2.1.2 Installation Requirements

Environment Space Power

At least 40 cm on each side, which is the preferred


access to perform service procedures

At least 10 cm behind for cabling and ventilation

There is enough room on or below the countertop to


accommodate the diluent, rinse and waste containers.
(reagents bottle better be the same level of the machine).
 

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2.1.2 Installation Requirements

Environment Space Power

 Check the availability of a power outlet that is


 a female receptacle;
 single phase with ground (confirmed third-wire earth ground);
 voltage of zero curve to ground should be ≤5V
 voltage: 100 VAC - 240 VAC;
 frequency: 50/60±1 Hz
 fuse: 250V T4A
 Better to have an UPS: 500W
 Properly grounded socket or connect the grounding pole at the
back of the analyzer to the ground.

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2.1.3 Installation Procedures
Release the sample probe Step2 Step3 Step4

1. Remove the foam


2. Move the sample probe to the middle position

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2.1.3 Installation Procedures

Step1 Reagents connection Step3 Step4

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2.1.3 Installation Procedures
Step1 Step2 Install Recorder Paper Step4

1.Open the door of the recorder.


2.Insert the paper into the paper entry.
3.Push the bar of the recorder.
4.Close the door.

Note: The thermo-side should faces up.

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2.1.3 Installation Procedures

Step1 Step2 Step3 Performance Validation

Start the machine

Check background

Do QC test,
check repeatability

Test fresh blood sample

Any step has problem,


please check the machine

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2.2 BC-2600 Operation

N
Repeatability good
Y Y N 2.2.1 Count Screen
CalibrationTroubleshooting 2.2.2 Menu Tree
2.2.3 Start up
2.2.4 Quality control
2.2.5 Sample and dispense
2.2.6 Linear and Display Range
2.2.7 Parameters

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2.2.1 Count Screen

System System
Title Count Mode Status time

Window

Error Messages Reagent Status


MENU On-line Help

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2.2.2 Menu Tree Count

Revi ew

Qual i t y cont r ol Cont r ol s

X- B anal ys i s

Set up Pas s wor d

Set t i ngs

Ser vi ce Mai nt enance

St at us

Sel f - t es t

Log

Conf i g

Pr i nt

Cal i br at i on Manual

Aut o

Aut o - f r es h bl ood

Shut down

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2.2.3 Startup

 After powering on the instrument, the power indicator will light on


and the screen will display “Initializing…”.
 The analyzer will sequentially initialize the file, hardware and fluidic
systems.
 The whole initializing process lasts about 3 - 5 minutes, depending
on how the analyzer was previously shut down.

 Background Test
Parameter Background Range
WBC  0.3  109 / L
RBC  0.03  1012 / L
HGB 1g/L
HCT  0.5 %
PLT  10  109 / L

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2.2.4 Quality Control

 Daily work: Before running any samples, use Quality Control or


Fresh Blood to check the stability of instrument.

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2.2.4 Quality Control

 Check CV : Run 11 tests and delete the first result,


other 10 results for analyzing.

n Parameter Linear Repeatability (CV%)


x i 4.0-7.5×109/L ≤ 3.0
Mean= i 1
WBC
n 7.6-15.0×109/L ≤ 2.0
3.0-4.5×1012/L ≤ 2.0

SD 
 X i  Mean 
2 RBC
4.5-6.5×1012/ L ≤ 1.5
n 1 HGB 110 – 180g/L ≤ 1.5
MCV 70.0 – 100.0fL ≤ 1.0
SD
CV%   100 100-200 ×109/L ≤ 5.0
Mean PLT
201 - 500×109/L ≤ 4.0

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2.2.4 Quality Control
 Good repetition, poor accuracy
Recalibrate
 8.9, 9.0, 8.9, 8.9, 8.8 (reference value: 9.6+0.5)

 Poor repetition, poor accuracy Troublesho


oting
 8.5, 9.4, 10.1, 8.0, 10.5

 Good repetition, good accuracy Perfect

 9.6, 9.7, 9.7, 9.6, 9.5


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2.2.5 Sample and dispense

 Compare
Blood Lyse

 Whole Blood mode 13ul 0.5ml


Prediluted mode 20ul 0.36ml

 Liquid tested Diameter of aperture


WBC/HGB 300ul 100um
RBC/PLT 300ul 70um

 Ratio of dilution Whole blood Capillary blood


WBC/HGB 1:308 1:400
RBC/PLT 1:44862 1:44402

 Aperture Size: 80um ( diameter ), 70um ( depth )

 Count time : less than 2 min / test


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2.2.6 Linear and Display Range

Parameter Linear Display


WBC 0 - 99.9 ×109/L 0.0-499.9 ×109/L

RBC 0 -9.99×1012/L 0.0-9.99 ×1012/L


HGB 0 - 300g/L 0- 300g/L

MCV ------ 0-250 fL

PLT 0 - 999×109/L 0-2999 ×109/L

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2.2.7 Parameters
Histogram Calculation Alarm
From Measurement

BC-2600can give out 20 parameters and 3 histograms


 Results coming from measurement

White Blood Cell WBC Red Blood Cell RBC


Lymphocyte Lymph# Hemoglobin HGB
Mid-sized cell Mid# Hematocrit HCT
Granulocyte Gran# Mean Cell (erythrocyte) Volume MCV
Lymphocyte % Lymph% Mean Cell (erythrocyte) Hemoglobin MCH
Mid-sized cell% Mid% Mean Cell (erythrocyte) Hemoglobin
Granulocyte% Gran% Concentration MCHC
Coefficient of variation RDW-CV
Platelet PLT Red Blood Cell (erythrocyte) Distribution
Mean Platelet Volume MPV Width Standard deviation RDW-SD
Platelet Distribution
Width PDW
Plateletcrit PCT
Large Platelet ratio P-LCR
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2.2.7 Parameters
Measurement Calculation Alarm
From Histogram

BC-2600 can give out 20 parameters and 3 histograms


 Results derived from the histogram
White Blood Cell WBC Red Blood Cell RBC
Lymphocyte Lymph# Hemoglobin HGB
Mid-sized cell Mid# Hematocrit HCT
Granulocyte Gran# Mean Cell (erythrocyte) Volume MCV
Lymphocyte % Lymph% Mean Cell (erythrocyte) Hemoglobin MCH
Mid-sized cell% Mid% Mean Cell (erythrocyte) Hemoglobin
Granulocyte% Gran% Concentration MCHC
Coefficient of variation RDW-CV
Platelet PLT Red Blood Cell (erythrocyte) Distribution
Mean Platelet Volume MPV Width Standard deviation RDW-SD
Platelet Distribution
Width PDW
Plateletcrit PCT
Platelet ratio P-LCR
45 © 2011 Mindray Confidential
2.2.7 Parameters
Measurement Histogram From Calculation Alarm

BC-2600 can give out 20 parameters and 3 histograms


 Results calculated by formula
White Blood Cell WBC Red Blood Cell RBC
Lymphocyte Lymph# Hemoglobin HGB
Mid-sized cell Mid# Hematocrit HCT
Granulocyte Gran# Mean Cell (erythrocyte) Volume MCV
Lymphocyte % Lymph% Mean Cell (erythrocyte) Hemoglobin MCH
Mid-sized cell% Mid% Mean Cell (erythrocyte) Hemoglobin
Granulocyte% Gran% Concentration MCHC
Coefficient of variation RDW-CV
Platelet PLT Red Blood Cell (erythrocyte) Distribution
Mean Platelet Volume MPV Width Standard deviation RDW-SD
Platelet Distribution
Width PDW
Plateletcrit PCT
Platelet ratio P-LCR
46 © 2011 Mindray Confidential
2.2.7 Parameters

Measurement Histogram Calculation


Parameters Alarm

 Alarm
“H” or “L” is indicating the data has exceeded the setup higher or
lower alarm limit of the parameter.
 
If the result is “***”, it indicates that the data is unreliable or has
exceeded the higher limit of the display range.
 
9
If the result of WBC is less than 0.5  10 /L, the system will not
perform leukocyte differential and shows “***”.

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2.2.7 Parameters

 Alarm
WBC differential alarm
R1 Alarm 
R2 Alarm
R3 Alarm 
R4 Alarm
Rm Alarm
PLT alarm
Pm Alarm
PS Alarm
PL Alarm

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2.3 BC-2600 Hydraulic System

2.3.1 Components2.3.2 Hydraulic Diagram


2.3.3 Counting circle

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2.3.1Components

Includes : 11 Valves(7 3-ways valves, 4 2-way valves) ,


aperture,
sample probe,
Syringe: 7.5mL & 50uL long life
Aperture size is Ø80um
pump, count bath, volumetric tube, wipe block,
vacuum/pressure chamber, tubing…

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Valves

Includes : 11 Valves(7 3-ways valves, 4 2-way valves)

When power on, When power on,


switch open

• Three way valve Two way valve

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Sample probe & bath

• Sample probe
Sample probe
• Rotatory motor assembly
• Fluctuating motor
• Counting bath
• HGB assembly

Counting bath
& HGB unit

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Syringe & motor

 7.5mL syringe
 50uL syringe Syringe
 Syringe sensor assembly
 Syringe motor

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2.3.1Components

 Vacuum chamber
 Waste pump

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2.3.2 Hydraulic Diagram

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2.3.4 Counting circle

Sample
Lyse
Diluent
Rinse
Waste
Air

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Aspirate sample (whole blood)

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Sample probe cleaning and
bath cleaning

Read HGB Blank

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Add sample, Draw sample(RBC)

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Aspirate Lyse, Add lyse ,Establish
pressure ,Bubble mixing

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WBC Counting

Read HGB Sample

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Clean bath

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Add sample(RBC)

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Establish pressure ,Bubble mixing, RBC
Counting

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Clean bath

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Aspirate sample (pre-diluted)

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Sample probe cleaning and
bath cleaning

Read HGB Blank

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Add sample, Draw sample(RBC)

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Aspirate Lyse, Add lyse ,Establish
pressure ,Bubble mixing

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WBC Counting

Read HGB Sample

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Clean bath

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Add sample(RBC)

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Establish pressure ,Bubble mixing, RBC
Counting

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Clean bath

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Clean-flush

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2.4 BC-2600 Hardware
2.4.1 Hardware Diagram
2.4.2 Board Structure
2.4.3 CPU board
2.4.4 Power supply board
2.4.5 Power driver board
2.4.6 Analog board
2.4.7 Volumetricboard

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2.4.1 Hardware Diagram

Old
version

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2.4.2 Board Structure

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2.4.3 CPU board

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2.4.3 CPU board

Power converter Part on CPU board

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Main function—CPU board
1. Receive signal from Analog board
WBC & RBC/PLT count signal
HGB signal
Aperture voltage
Pressure/vacuum
2. Monitor
+48V 、 +12V 、 -12V from analog board
+3.3V 、 +12V from CPU board
Temperature
3. Control
Keypad
Valves & pump
HGB lamp
Constant current
Potentiometer
4. Drive
LCD display
Keyboard
Printer
Floppy disk driver

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2.4.4 Power Supply board

Input power 100~240V ,


Output 5V 、 12.8V 、 30V 、 ±12V
 3 power lines to CPU board, analog signal board
and power driver board. CPU board supplies power to
other boards.
old version 2600:A transformer converts AC input
power to AC53V(converted to aperture constant
current 48VDC) and AC120V(ZAP function)
New version: these circuit are on analog board

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2.4.5 Power driver board
Input power from power supply board (12V 、 30V)
The driving board drives the valves, pumps and motors.
It carries out the following instructions sent by the CPU:
to open/close the pumps or solenoid valves;
to control the motors of the syringes;
to control the movement of the sample probe;
to remain the torques of the motors when the analyzer has
entered the screen saver.

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2.4.6 Analog signal board

Power board supplies ±12V


Old version

Basic functions:
 Power supply unit:
 Volumetric signal adjustment unit;
New version  HGB signals adjustment unit:
 Vacuum/pressure signals adjustment unit:
 Monitor unit: monitor operating voltage and the
volumetric signals sensor.
 Interface unit: interface of analog board and CPU
board.

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2.4.7 Volumetric board

Control the count cycle. Each count cycle starts


when the liquid surface reaches the upper
sensor and stops when the surface passes the
lower sensor.

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2.5 System Structure

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Overview

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Removing the left, right and top covers

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Removing the front cover

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Disassemble boards

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Syringe

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Counting Bath

Note: The concave side of the aperture must face to


the front bath.

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Counting bath

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3-way valve

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3-way valve

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2.6 Trouble shooting
2.6.1 Adjust Gain
2.6.2 Histogram and pulse graph
2.6.3 Trouble shooting

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2.6.1 Adjust Gain

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General

Adjust the Gain of the Channel in the Following


Situations:
 Replace count bath

 Replace aperture

 Replace analog signal board, CPU board, DOM

WBC (whole blood), WBC (pre-diluted), RBC, HGB and


PLT

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Gain of WBC Channel

 too small WBC gain that should be increased

 too large WBC gain that should be decreased

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Gain of WBC Channel

 Use normal volunteers’ fresh EDTA-K2 anticoagulant


venous blood;
 Make the end position of histograms of most blood
samples between 320-350fl;
 Set up WBC (pre-diluted) gain the same as the WBC
(whole blood) gain.

100 © 2011 Mindray Confidential


Gain of RBC/PLT Channel

 When the difference between the actual MCV


analysis result and the expected result exceeds
6%, need to change the RBC gain.
 For example, assuming the expected MCV result
is 90.0fL, while the actual analysis result is 82.0fL,
the gain factor as below:
ExpectedMCV 90.0
=  1.098
ActualMCV 82.0

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Gain of HGB Channel

• Modify the background voltage value of HGB


within the range of 3.4-4.8V (4.5V recommended).

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2.6.2 Histogram and pulse graph

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Normal histogram

Fluctuating
Discriminator WBC 1 2 3 4
1 30~85fL
2, 3 85~125fL
Fixed Discriminator
4 400fL

[fL]
Lymph Mid Neutro
(Baso 、 Mono 、 Eosino)

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No differential result

No differential result because the WBC histogram is


over-narrowly compressed.

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No differential result

No differential result because WBC count result is less than a


certain value (WBC < 0.5)

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No differential result

No differential result because the peak of WBC histogram


lies in the middle of the histogram and thus cannot identify
the type of peak cells

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Increased nucleated erythrocytes or interference or inadequate
lyse

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No lyse reagent or poor lyse

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Severe interference in WBC channel

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Increased lymphocytes

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Increased mid-sized cells

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Increased neutrophilic granulocytes

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Pulse Graph

 After each count, the system can save the original


sampling pulses of this time. We can analyze the
reason leading to the fault by viewing these
original data.  

 For BC2800, please press F5 to view WBC pulse


and press F1 to switch to RBC and PLT pulse
graph.

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WBC pulse Graph of normal sample

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WBC pulse graph of normal background

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RBC pulse graph of normal sample

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RBC pulse graph of normal background

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PLT pulse graph of normal sample

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PLT pulse graph of normal background

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Severe interference in WBC channel (background)

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Severe interference in WBC channel (sample)

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Severe interference in RBC channel (background)

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Severe interference in RBC channel (sample)

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Severe interference in PLT channel (background)

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Severe interference in PLT channel (sample)

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Interference occurs because gain of PLT channel is too large

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Slight interference in WBC channel

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Inadequate or no hemolysis in WBC channel

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Slight interference in RBC channel

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Slight interference in PLT channel

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Interference in WBC channel caused by inverter
Feature: sine wave

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Interference in WBC bath from inverter

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Insufficient liquid in WBC bath during count

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Interference in RBC channel from tubing

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Insufficient liquid in RBC bath during count

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Interference in PLT channel from tubing

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Insufficient liquid in PLT bath during count

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2.6.3 Troubleshooting

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Background abnormal

Background
abnormal  Not well grounded
 Shielding cover of bath and analog board
WBC(RBC) clog
 Electrical interference
WBC(RBC) bubble  Reagents dirty
Vacuum low  Bath, valve or tube dirty
 Wipe block, diluent dispenser, shielding cover,
Pressure low
 7.5mL syringe dirty
Results abnormal  Plastic washer dirty, aging
WBC abnormal  Replace bath
 Replace analog board
Syringe error
 Pulse graph
Display problem

No start up

140 © 2011 Mindray Confidential


WBC(RBC) Clog

Background
abnormal
 Reagents empty
WBC(RBC) clog
 Diluent added into the bath sufficiently
WBC(RBC) bubble  Zap, flush
Vacuum low  Aperture voltage: 9~14V, if high, unclog
 Watch volumetric board
Pressure low
 Fluid pass normal, volumetric board defective or need
Results abnormal adjustment, CPU board
 Fluid pass abnormal, check valve 6, tube between valve 6 and
WBC abnormal
bath, valve 6 to volumetric tube
Syringe error

Display problem

No start up

141 © 2011 Mindray Confidential


WBC(RBC) Bubble

Background
abnormal  Reagents empty
WBC(RBC) clog  Diluent prime, rinse prime
 Diluent added into the bath sufficiently
WBC(RBC) bubble
 Watch volumetric board
Vacuum low  Fluid pass normal, volumetric board defective or need

Pressure low adjustment, CPU board


 Fluid pass abnormal, check valve 6, tube between bath to
Results abnormal
vacuum chamber leak or not.
WBC abnormal

Syringe error

Display problem

No start up

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Vacuum low

Background
abnormal  Service->System status->Vacuum
 Vacuum filter
WBC(RBC) clog
 Waste container full
WBC(RBC) bubble  Waste tube bent
Vacuum low  Tube connections between valve(6,7,8,9,10)
 Valve(6,7,8,9,10)
Pressure low
 Waste pump block, clean
Results abnormal  Vacuum chamber leak
WBC abnormal  Tube between vacuum chamber and analog board
 Analog board
Syringe error

Display problem

No start up

143 © 2011 Mindray Confidential


Pressure low

Background
abnormal  Service->System status-> Pressure
 Wipe block blocked
WBC(RBC) clog
 Tube connections between pressure chamber,
WBC(RBC) bubble pressure pump, valves, analog board
Vacuum low  Valve 8,9
 Vacuum pump
Pressure low
 Analog board
Results abnormal

WBC abnormal

Syringe error

Display problem

No start up

144 © 2011 Mindray Confidential


Results abnormal

Background
abnormal  Check blood sample
 Check reagents
WBC(RBC) clog
 Check background
WBC(RBC) bubble  Do QC test
Vacuum low  Use control to check CV
 Calibration
Pressure low

Results abnormal

WBC abnormal

Syringe error

Display problem

No start up

145 © 2011 Mindray Confidential


WBC abnormal

Background
abnormal  Check lyse
 Lyse test
WBC(RBC) clog
 Lyse not enough, check valve 6 and relevant tube
WBC(RBC) bubble  Lyse enough, then
Vacuum low  Check background
 Check repeatability of WBC, good then calibrate
Pressure low
 Repeatability of WBC not good, check relevant parts
Results abnormal WBC bath, valves 1,3, tubes, analog board
WBC abnormal

Syringe error

Display problem

No start up

146 © 2011 Mindray Confidential


Syringe error

Background
abnormal  Service->Self Test-> 7.5mL(50uL) syringe
 Remove tubing connected to syringe, then test again
WBC(RBC) clog
 Add lubricating oil to axis of motor
WBC(RBC) bubble  Syringe sensor dirty or loose
Vacuum low  Disassemble and clean syringe
 Check optical sensor cable, motor cable to power driver board
Pressure low
 Optical sensor defective
Results abnormal  Motor defective
WBC abnormal  Power driver board defective (other motor might have error
too)
Syringe error

Display problem

No start up

147 © 2011 Mindray Confidential


Display problem

Background
abnormal  Check and adjust backlight, contrast
 Check cable between LCD screen and CPU board
WBC(RBC) clog
 No backlight? Converter board defective?
WBC(RBC) bubble  Replace the cable
Vacuum low  Replace LCD screen
 Replace CPU board
Pressure low

Results abnormal

WBC abnormal

Syringe error

Display problem

No start up

148 © 2011 Mindray Confidential


No startup

Background
abnormal  Power, fuse
 Disconnect all boards from power supply, use multimeter to
WBC(RBC) clog
measure output
WBC(RBC) bubble  Only connect CPU board, analog board, if no start, power
Vacuum low supply board, CPU board, DOM
 Remove motors, valves, pump from power driver board
Pressure low
 Connect CPU board, power driver board, if no start, power
Results abnormal driver board defective
WBC abnormal  Short circuits in valves, pumps, motors

Syringe error

Display problem

No start up

149 © 2011 Mindray Confidential


2.7 Maintenance

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Daily maintenance

If it works day and night or the sample volume is more than 100
per day, execute “E-Z cleanser cleaning” operation once every
day.

If the quantity of samples more than 50pcs, set the “Auto clean
time” 4 hours.

If the user turns off the instrument every day, the user should
execute “probe cleanser cleaning” operation once every three
days. Or do it when the machine remind you.

Use E-Z cleanser to execute shutdown program.

Dispose the waste liquid

151 © 2011 Mindray Confidential


Monthly maintenance
 Use probe cleanser to clean the sample probe wipe
block.

 Use sample probe localizer to correct the position of


the sample probe.

 Use E-Z cleanser or probe cleanser to clean the


baths.

 Calibrate the unit.

152 © 2011 Mindray Confidential


Adjust the sample probe position
Procedure
1. Take off the right side panel of the equipment.
2. Input password, then access the “self test” menu in “service”
3. Press [F1] and select the “mechanics” press [↑]and [] to drive
the motor to the upper place of counting bath.
4. Move up the sample probe to the end
5. Loose the screw that fixed the probe
6. Using localizer to correct the probe and then tighten the screw

Localizer

153 © 2011 Mindray Confidential


Half-year maintenance

 Replace vacuum filters.

 Test all valves’ work status.

 Clean the valves, especially the V8, V9,V10.

 Disassemble and clean the two baths and


apertures.

 Check the stability of HGB blank voltage.

 Check the stability of vacuum.

 Clean inside of the equipment.

154 © 2011 Mindray Confidential


Version Information
Version Author Date Description
1.0 Rany 7/11/2012 First Version
2.0 Rany 2/10/2014 N/A

155 © 2011 Mindray Confidential


Check Information
Version Reviewer Date Comments
1.0 Carl 7/13/2012 N/A
2.0 Mars Zhang 7/10/2014 Approved

156 © 2011 Mindray Confidential


Thank you!

157 © 2011 Mindray Confidential

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